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Question #1:

Calculating enzyme activity (Beer-Lambert Law etc)?

We did a lab on 'Effect of pH/temp/[S] on enzyme activity' using alkaline phosphatase. I understand all of the calculations except for one bit:

After the Beer-Lambert Law we need to calculate the amount of pNP in the tube, this is directly from our sample calculation:
c = .500/18300 x 10^9 nmoles in 1000mL
? in 4mL

Where the hell did 4mL come from?? But then in the sample given to us for the [S] activity, we were given 3mL. I have no idea where these volumes are coming from, please help!
Woops, I thought I did include info about the experiment, I must've forgotten:

We had 10 tubes, 5 tests and 5 blanks. Each tube 1-5 had a different pH and 1.0mL of 7.5mM substrate (pNPP).

We added 1.0mL alkaline phosphate to the tests. After incubating, we stopped the reaction by adding 1mL 2M NaOH to the tubes.

We added 1.0mL 2M NaOH to the blanks. Then added 1mL of alkaline phosphatase to the blanks.

Sample Calc:
a=Ebc
.500 = 18300 x c x 1cm
c = .500/18300 M

c = .500/18300 x 10^9 nM
ie .500/18300 x 10^9 nmoles in 1000mL
? nmoles in 4mL

=.500/18300 x 10^9 x 4/1000 nmoles produced in 10 min by 1 mL enzyme

=.500/18300 x 10^9 x 4/1000 x 1/10 nmoles min^-1 mL^-1

=10.0nmoles pNP
Oh and the tubes also had 1.0mL of buffer.

Question #2:

Why am I a recluse? and what can i do to change that?

I moved to BC to live with my best friend. i had not seen him in 6 yrs and rarely spoke however we were still best friends. he came back to my city, and came to see me, it was like we were never apart. He was the kind of person who would support anything youd do. I ended up moving to BC with him. we became super close again, we went out and he showed me Vancouver and the best times of my life. We started to sell weed so we wouldn't have to pay for it and things were great. we made money and the people we worked for loved us. one day they asked me to take weed to calgary on a bus and i agreed. the day i was leaving was a great day. me and my friend laughed and enjoyed our day together. i had to leave at 5am and was hanging out at our friends house. my best friend went to go get my luggage. he said he would be 15 mins and asked if i wanted to go with him. i said no. he never came back. the police found him on the street dead. the police did not believe me and i was apprehended.i was interrogated and told them everything, and was released. i had to borrow money from my mother to get a bus ticket home. when i returned to my home town. everybody blamed me for my friends death. i had people throw full beers at me from cars only to hit my gf in the head. she has left me due to it. all my friends that i used to have, abandoned me, they dont answer my calls. i gave my friends eulogy at his funeral, and his dad and his uncles cornered me outside and threatened me. his cousin saw me and tried to run me over with his car. he drove up on the curb and jumped off my bike to not get hit. ive called the cops on his family for threatening me. its to the point where i am afraid to go outside due to the way people interrogate me over and over. its been a little over a year and since then i have given up on society. i joined the navy so i could get away ASAP. i cant even walk to the store without people harassing me. i worked at a restaurant and his father showed up he travelled from b.c. just to harass me some more. no girls will even talk to me, no guys will let me walk down the street in peace. i have no friends. I am alone in this world, everyone either interrogates me or makes fun of me saying im crazy. sometimes i just want to disappear and never look back. when i do try to go out i get such bad anxiety i have had to deal with guilt that others have placed upon me. i am so sad. my family doesnt know how to help me. why cant i get over my best friends death. i feel like if i do make new friends i am scared to get close to them, losing a best friend is one of the hardest things i have ever had to deal with. his murder was a hit and run. everybody thinks i did it but i have people who were with me at my friends house. I always was popular, i was always happy. how can this one tragedy effect me for so long. when will it get better. will i have to be depressed for the rest of my life? is there anybody who can help me? I dont understand why i have to go through this alone. and how my friends could just abandon me at my time of need. since my friend was killed i have gone to college and graduated with honors, i have quit smoking marijuana and i dont drink or smoke cigarettes anymore or even watch tv or play video games. all i do is workout and prepare for going to africa with the navy. i have no social life and im not the same person i used to be. i was the life of the party before. now im just another emo kid... will i ever be able to hold my head up high again? if you met me you wouldnt be able to tell that i was so depressed i hide it well... i just want a girlfriend which was never hard for me to do.. im good looking and i take care of my body, i always have a job and work hard on my future... but my confidence is not what you would think anymore... im a sad case now... i ahev been single withouit even a girl giving me her phone number for almost 2 years... girls sometimes ask me why i am single and i just dont know what to say... psychiatrists dont help, Councillors havent helped... and even the police special victims people cant help me... i just want someone who loves me to tell me everything is going to be ok... im leaving for africa in january and nobody even cares i tried to have a going away party and nobody showed up... like why do i even bother... can someone please give me some advice.. i take vitamins and workout daily.. i go swimming and take a sauna everyday.. i run alot. but exercise and proper eating can only do so much... I hate the way my life has turned out... even though i am successful now i still feel like life is pointless... i am not suicidal... i just dont know how to deal with the loss of my absolute best friend... why did he die and not me. why didnt i go with him... why do i have to deal with this stuff. all these questions i ask myself everyday and i know it is not healthy. and this can happen to anybody if you sell marijuana or not.
that felt great just getting it off my chest... nobody will ever listen to me, so even if nobody answers me it felt great to just rant like somebody out there cares
I am staying with my only family.. my mother who has been my rock in the raging river that is my life... i dont know if i can wait until january.. its so hard... do you know about grief councilling?
thank you humpty dumpty i appreciate your delicacy in addressing the situation... i know yahoo answers isnt the best place to get these types of answers but im very pleased to see such compassion in answering.. thank you so much for listening

Question #3:

I ratted out my friends for drinking beers?

they were drinking beers in the school yard. they wouldnt let me join them. I called the cops on them. I did the right thing didnt i?

Question #4:

Who wants to read my nagging story?

Just met a guy last week. Started hanging out for the first time a few days ago, and I let him know right off the bat that I wanted to take things slow, and do things the right way. So, the second time we hang out..things were cool, i went home and he texts me like an hour later, drunk and telling me he wants me to come over and watch a movie with him. Again, i explained to him i didnt want to ruin anything because hello, we all know he wanted more than just me coming over to watch a movie with him. Especially when he was drunk. Anyway, third time we hang out....i meet up with him and his guy friend at the movies. A couple of cute females enter the theatre, and the guy im with is staring them down and elbowing his friend to check them out. I thought that was kind of rude especially when im supposed to be his date..but i ignored it. After the movie, he wants me to follow him in my car to a place thats 30mins from where we are at, and i asked if it was okay if i rode with him enstead to save gas. Well he gives me the strangest look, as though i was an inconvenience then says "ummmmm...okay you can ride with us." So we get to his friends place and play a few rounds of beer pong, and he says to me "you're going to have to stay the night at my place, because i dont want to drive if im getting drunk." Neither of us got drunk that night...and so basically he was too lazy to take me back to my car, and i ended up staying the night. He tried sleeping with me, but i turned him down. This guy also has never really been in a long term relationship with someone..so theres another red flag. do you think i should drop him or give him another chance? Keep in mind, i've only hung out with him a few times (on dates) and he already wanted me to be his girlfriend. Also mentioned having kids. Dude is moving wayyy too fast.
and thanks for the mature people that actually answered this and werent d***s like the other people on here.

Question #5:

Survey: This or That (fun stuff)?

1. Rap or Screamo?
2. Glasses or Contacts?
3. Brown or Black or Red or Blond hair?
4. Pepsi/Coke or Sprite or Root Beer?
5. Sushi or Curry?
6. Blue or Green?
7. White or Black?
8. =) or :) or (:
For me its:

1. Rap or Screamo? - screamo all the way!
2. Glasses or Contacts?- I have glasses.
3. Brown or Black or Red or Blond hair? black/ brown, i have black
4. Pepsi/Coke or Sprite or Root Beer? -coke
5. Sushi or Curry? - curry i guess
6. Blue or Green? - I like both but ill choose blue
7. White or Black? Again I like both but ill choose black
8. =) or :) or (: - :)

Question #6:

question about liver cancer?

how often is it terminal? The doctor said he better pray, but never used the word terminal, he has drank beer for 50 years, which hurt his liver. what are the chances its terminal?

Question #7:

Need advice in Delaware law for underage possession of alcohol!! Please?

I was recently stopped by a Newark police department cop who saw me walking with an open bottle of beer in my hand. I was written a citation for underage possession of alcohol and given a court summons date. I was told the fine would be for about $200 and I would have a criminal record. I have no problem paying the fine but do not want to have a criminal record as it could hurt with job possibilities in the future. Should I attempt to fight this case? Will pleading not guilty get me off the hook if the cop is a no show on my summons date? What are Delaware consequences for first time offenders charged with underage possession of alcohol? Is there anyway I can get this criminal record removed or expunged? And if not, will it ever disappear? please not that i am also 18

Question #8:

do you like poetry with puppy dogs and doves?

why, Daddy?

well Sweetheart, once upon a time....in a village where everyone was nice, a mean old witch, brought the village people, a gift in a basket. Now, because she was a mean old witch, no one wanted to look in the basket, fearing something bad might be in there. So they took the basket into the woods and called S.W.A.T., knowing they were the best to handle this problem.

S.W.A.T. came and sent in a mechanical robot, with lots of eyes and metal arms and no heart. You know, just in case there was something bad in the basket. Soooooo, the robot approached the basket and with it's non-human heartless arms, it opened the basket and set an anti-bad thing device inside and backed away and waited.

All of a sudden, BOOM !!! There was a huge explosion and lots of fur and feathers and internal organs and fluids went everywhere.It was an awful sight. The old witch knew they would not trust her, so she filled the basket with puppy dogs and doves.

Then the S.W.A.T. people left and went to O'Malleys and drank beer and laughed.

but why, Daddy?

Question #9:

please help me with your opinion on this 10 points to best answer!?

Last friday I went to a football game with my brother-in-law cousin (not blood related). we drank some beer at the game and all, anyway after the game her aunt was picking us up since we couldnt drive home i had my arm around her shoulder at that time. My brother in law cousin works at a bar so she asked me if i wanted to go chill with her there and drinks ome more (after all free drinks for me). As time passed I was talking to some old friends i saw there and my brother in law cousin name is mindy so mindy sat next to me at the bar saying i been looking everywhere for you matt (My name).

we sat next to each other she every now and then touched my arm when we were talking and i put my hand on ehr knee and she didnt move it, a few minutes later we danced all i remember is that she was grinding on me and i had my arms around her at the same time. As time passed i was wasted lol so mindy bought me back to my brother in law house since i was staying there for the night. we pulled up and i couldnt make it up the stairs so she helped me and helped me get my pants off ( not the sexual way) i laid down on the sofa and i asked her to come by me and she said she had to go back bc she got asked to work so i waved my arm for her to come by and she did and she said thank you for coming to the game with her and i gave her a hug and a kiss on the cheek.

Last night I went out with her again to the bar and this time i drove as we were walking to my truck I put my arm around her shoulder then i moved it down to her waist and she didnt say or do anything. I slept by her place on the sofa and her in her room but before we went to bed she said the tv remote is here you can watch whatever and i said i cant bc your but is in my way and i gave it a light slap and i said laughing what that a jiggle? and she laughed (we were a lil tipsy). She is the type of girl who goes out to the bars almost everynight and sees her friends guys and girls and they kiss her on the cheek so i dont think she is the shy type. I dont want sex right now bc i like to take things slow but I really want to kiss her or her to just lay a kiss on me. does she even seem interested in me in anyway???? I would love a kiss from her why doesnt she kiss me if she is interested? i think that she is just being nice to me and is afraid to let me know and make me feel down. she doesnt get drunk easy so she was sober

Question #10:

Why do men like beer, but generally women don't?

i think every guy i've ever met has loved a good cold beer, but i haven't known many women to enjoy sitting down and sipping a pint.

why might this be?

Question #11:

Old friend visiting for lunch soon... HELP!?

Ok so I just recently got in touch with my old basketball coach who has been a really cool guy. I am alot older now (22) and I guess he's like 30 or 40ish. Now he invited me to lunch to catch up and stuff and he is like sorda rich, but at the same time hes really down to earth and I think he loves like every sport! So my question is should I bring like wine or something,or beer. I don't want to come empty handed because I don't want to appear childish or anything, but beer I was thinking because he's a cool guy, but then wine is like proper and I figured it would show maturity, Am I on the right page, any other things I could do? Thanks guys!!!

Question #12:

If I pour beer and whiskey all over my iPod, will I get free Ke$ha songs?

Not that I want them. I'm just curious.

Question #13:

My uncle is scaring me...?

I'm really scared of my uncle now. please read this!
Okay, i came to visit my grandmother in Thailand and all my cousins and aunts and uncle lives there.
and well, my cousin has school and my aunts have work, so every sunday and monday and tuesday and ect..., they go out to work. But my uncle, he's taking a break from work, so im left alone with him and my sister. Me and my sister were really bored so we were talking to him and stuff and playing on his phone, (cuz my phone ran out of battery and i forgot to bring the charger). He became really close to us. So the next day we went into his room and played on his phone/ watch tv with him.
But the day after that, he kept saying i was 'beautiful' or 'very pretty' and he would stroke my hair. I became VERY scared, so i just nodded and said 'yeah yeah' and walked away. the next day i stayed in my room the whole day watching tv, but my sister however was in my uncle's room. i went to check on her. She was lying on the bed next to him watching tv, he was massaging her legs and arms, and her skirt was up so you could see her undies. I got soo pissed at her for letting him touch her. She's only 8, and after she left his room i told her not to let him touch her again.
I'm only 13 >.<

my uncle is like an old perv... he drinks beer everynight and he's really lazy so he would use his son to get him everything. And i went through his phone and he has like all these porno -.-
i know its ordinary for men, but i am so scared of my uncle.
i havn't told anyone yet and i dont want to. Whenever he walks through my room he keeps glancing at me. its so freaky. I'm scared he's gona do something to either me or my sister.

I swear. Please help me D: !

Question #14:

Has football become tribalism rather than actual interest in the games themselves?

Tailgate parties, beer drinking, camaraderie among guys, a kind of tribal connection to our paleolithic ancestors. Is this what football has become? The novelty has long since worn off as far as anything new you can see in a football game that you haven't seen before. Players have peaked out as far as ability. Nobody can be any better than Peyton Manning, Barry Sanders or Jerry Rice? I can't even watch more than 1/3 of a game anymore. I very seldom watch a game before the fourth quarter. Thoughts on the tribalism aspect of football and I very much include college football in that description as well?

Question #15:

I am going to give up with being sober... How much alcohol is enough?

Short background, I am 23 years old (male), 6'0", 190lbs. I haven't had an alcoholic beverage since I was ~ 16 years old.

I have never been to a bar, or any night club like situation since like high school. Even in high school, when I drank, I only had a beer or something.

I went to college and my first year, I made no friends except for a few guys. None of them ever went to bars, so I never went. They drank, but I didn't.

I then transferred to another school, and made even less friends (as pathetic as it is, I went 3 years without a single person (besides a few "business" contacts) giving me their number. I never went out.

As you can see, I really have never had a reason to drink, (unless I was to drink alone in my apartment).

What started out as "well, I just never had a reason", became... "well i haven't drank in 7 years, why start now".

I am a first year medical student now, and tried to open up and make some friends. It worked, and around 4 girls and one guy, said they were going to the night clubs in downtown Seattle. They pressured me into going. I said "well i don't drink"... Please note, i had no reason not to (until later that night, shortly before going out, when they found out i don't drink and i became the DD).

I felt really awkward and uncomfortable. I am not going through that again.

I am going to drink (hopefully I can control myself =/) next time I go out like that. How much is just enough to loosen me up, and get rid of that nervousness/ uncomfortableness.

Question #16:

I am going to give up with being sober... How much is enough?

Question #17:

Drinking whilst on Beta Blockers?

Hello everyone
I’ve recently been given Propranolol to help with my anxiety and the physical effects that comes with it.
The medication greatly helps me in that aspect and I’m happy about this.

Problem is this is the first anxiety meds I’ve ever taken and the advice booklets that comes with it says to avoid drinking with them if possible.

Im not a big drinker anyway, maybe a couple of bottles of beer a week, I was wondering if it’s ok to have a couple of bottles on social occasions with friends or if it should be avoided completely?

Question #18:

Is mixing beer and rum a bad idea?

if yes why? do explain

Question #19:

Please tell me what you think about this :)?

So this is a fiction story i just finished recently.this is part of it, let me know what you think. Oh, and this is about a girl and a guy she loves. he gets with his ex while she is gone and she is heart broken. ANd he calls her Angel because he sees her as just a beautiful thing and thinks she's his angel.
“Angel,” someone said. I jumped and began to slip off the rock. I tried to grab something, but the rock had no cracks in it. I was doomed and I was going to fall onto all those other little rocks below.
A hand grabbed my arm and my waist and pulled me onto my back. “Dammit! Don’t just pop out of no where like that!” I said and hit Claude’s arm. He chuckled. “Your welcome,” he said eyeing me. Oh...that’s right. “Thank you,” I told him.
He was the last person I wanted to see right now. Because every time I looked at his face, the pain would come back, not hard, but I would feel his betrayal and false love.
Gosh! I couldn’t get a hold of myself, even if my life depended on it. One minute I would think he didn’t matter but the next I would be wanting to fall into his arms.
I looked away and up at the sky. “What are you doing here?” I asked. “What I’m not welcomed?” he asked. I laughed softly. “No, I mean. Why aren’t you having fun with the others?” I asked. “They’re getting crazy over the games. So I wanted to come get some peace,” he explained nonchalantly.
I didn’t say anything. I began to count the stars, I always did this when I wanted to avoid something. Count.
“Why aren’t you over there?” Claude asked. I stopped at fifty-seven. “I don’t really enjoy being around many people, especially one’s I don’t know,” I said and began counting again.
Fifty-eight, fifty-nine, sixty, sixty-one, “Well then you should go meet new people. Thats what this stuff is for,” he said. I groaned. “Thanks a lot Claude! I lost count,” I said frustrated. He laughed. “Sorry. You should have told me you were counting stars,” he said. I looked over at him.
He was grinning. Then brought a bottle to his lip and drank. My eyes widened. “You drink?” I asked. He but the bottle down. “Only one,” he said and shrugged. “Claude you never told me you drank!” I yelled. He laughed. “This is nothing. I drink wine too, but I don’t get drunk,” he said the last part sternly. I shook my head.
“Wow, there’s a whole side of you I don’t know,” I told him. I couldn’t believe my eyes. It’s like the Claude, my sweet Claude changed before my eyes.
“Adela, when you were with the band, it was only like a week. You need to spend a lot more time with me if you want to know me like the boys do,” he said and looked at me right in the eyes.
He was stupid! Does he think I have all the time in the world for him?
I snatched the bottle from his hand. He looked at me quizzically. I poured out the beer and let the ocean wash it away. “Adela,” he said. I handed it back to him. “Well, I don’t want you drinking. Got it?” I asked. He looked at me like I was boring.
“I thought you were going to drink from it,” he told me. I scrunched my face. “Heck no. You kiss Stella, so that means you guys share the same saliva, and I don’t want her saliva in my mouth,” I said annoyed. He laughed. “That doesn’t mean anything. I kissed you,” he said.
I jerked. I knew it. It didn’t mean anything. Huh. The way he just brought the subject up. This guy was alien to me.
“It means a lot,” I told him and looked at him angrily. He looked shocked. “I didn’t mean it like that angel,” he told me sternly. “Don’t call me that,” I told him.
There it is. please be nice i am new at this. :) Thanks for you time! Tips would be awesome!
MChannn, Thank you! I am 14 as well i really appreciate the example you gave. The reason I don't give descriptions is because this isn't the beginning it' like in Chapter 20 something. LOL so sorry if it was confusing. Thank You SOO much and have an awesome day! :)